Classify and Quantify 2D Droplet Digital PCR Data with twoddpcr Package

The twoddpcr package is a groundbreaking open-source tool that streamlines the automatic classification of two-channel droplet digital PCR (ddPCR) data. This innovative software enables users to generate a summary of positive and negative droplet counts, which can then be used to estimate the number of molecules present using the Poisson distribution.

The twoddpcr package is specifically designed for use with Bio-Rad's QuantaSoft, allowing users to classify droplets based on their amplitude data. This package is particularly useful for researchers who need to constantly download and analyze large datasets.

To get started with twoddpcr, users can export their CSV files from QuantaSoft using the 'Export Amplitude and Cluster Data' link. The software will then prompt users to select a directory for exporting the files. Once loaded, the data must be exported as CSV files.

For optimal performance, it is recommended that users utilize laptops specifically designed by Bio-Rad for the QX100 or QX200 ddPCR System. Additionally, users can export their QuantaSoft data table as a secure PDF for enhanced security.

The twoddpcr package offers a range of benefits, including efficient classification and quantification of 2D ddPCR data. With its user-friendly interface and robust features, this software is an essential tool for researchers working with Bio-Rad's QX200 Droplet Digital PCR System.

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